The overall objective of this experiment was to see based of our initial experiment if making adjustments the concentrations would allow the dye to move into the cell through simple or facilitated diffusion. The results of the experiment did not support our hypothesis that by increasing the NR-Dye concentrations the dye will diffuse through the cell via facilitated diffusion. Instead it was observed that cells diffused through simple diffusion. In the graph, which was for our DIY experiment we can see that as we increase the concentrations the absorbance of the dye by the cell increases as well. In replication 1, it can be seen that around 1.25% concentration the absorbance goes straight up at an exponential rate, making the results obtained for Rep.1 wrong due to an experimental error that could have occurred when transferring the samples from the microfuge tube to the microtiter plate. For Rep. 2 & 3 the absorbance begins to level off as the concentrations get higher, we would have liked to conduct more replications and see if after a certain amount of replications at high concentrations how much of the NR-Dye the cell would let in. An experiment was performed which used
grown in the presence of 1.0% glucose, and their results showed as the glucose concentration increased, metabolic activity and catalase functions decreased (Alm and Friedman, 1962). They also indicated the pH level when acidic lowers the metabolic activity and suppresses enzyme function. Our experiments pH was measured at 6.8 in the beginning of the experiment. In a future experiment, the pH can be considered more.
In conclusion, the Nr-Dye required no energy to diffuse through the cell membrane.