Introduction: specific pathways on cancer cell region


Chemotherapy in combination with
nanotechnology as nano-chemo-therapy provides a wide range of drug formulation
perhaps it provides a specific therapy over the cancer region (Bhirde et al., 2009), high efficacy, safety
& minimum side effects over the conventional chemotherapy (Allam et al., 2017). The unique design of nanoparticles
provides a platform for dispensing the
cancer drug through specific pathways on cancer cell region with a minimum side effect on normal cells (Jenning et al., 2000). The use of polymeric
nanoparticles is providing a controlled release of drug specifically over tumour cell & widely accepted drug delivery
system against cancer to diagnosis and drug therapy. Nanoparticles within a
drug capsule create a great potential to demonstrated and enhance controlled
drug delivery system (Mohammad et al
2015). Polymeric nanoparticles with chemotherapeutic drug molecule developed a
conjugate/encapsulated carrier system for newer nano-chemotherapy and
polymeric-nanoformulation provides a submicron size (1-1000 nm) of colloidal
drug particles with multifunctional/multi-component drug system over the cancer
treatment. In this drug therapy, the
therapeutic agent can be able to encapsulate in this bunch of matrix and get
absorbed on the surface. Now’s days nanoparticles are using as drug delivery
vehicles with different biodegradable polymers for the enhancement of efficacy,
half-life, targeted delivery thereby
increase the therapeutic index of particular drug system (Liu et al., 2012). 5-fluorouracil (5-FU) is a chemotherapeutic agent for an anticancer activity, it is an analogue of pyrimidine uracil which occurs naturally (Ma et al., 2014). Clinical metabolism of 5-FU indicates that the
anabolic pathway inhibits in between the
methylation reaction of deoxyuridylic
acid to thymidylic acid. The pathway of 5FU activity is interfering the DNA
formation and so that indirectly its growth and its division and resultant
death of cell occur because of thymine deficiency (Bocci et al., 2006). The conventional dosage of 5-FU has the short
half-life, less bioavailability, non-specific drug delivery and other many adverse
effects. (Nair et al., 2011). In addition
with drug delivery to any molecule like 5FU with nanotechnology leads to minimizing
their limitation such as short half-life,
toxicity, and non-selective action
against cell by the unique properties of nanoparticles with nano-size dual/triblock structures (Oh et al., 1999). During the research on the classical antitumor
entity 5 FU, the pharmacokinetic profile is needed of the formulated novel
polymeric nanoparticles (Matteucci et al.,
2006). Our study is mainly focused to develop the polymeric 5-FU nanoparticles
and validation of analytical data to collect the complete drug profile of the
drug. HPLC-UV (high-performance liquid chromatography-ultraviolet) is very much
reliable and classical technique to identify
the quantitative analytical method development and validation (Kumar et al., 2014).

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Material & Method:

& Reagents: 5-Fluorouracil (5-FU)
Extra (Cas No. 51.21.9) was obtained from Sigma Aldrich, PLGA, Polyvinyl
chloride, Megestrol acetate, Acetonitrile & methanol, Extraction agent
ethyl acetate, other chemicals & solvents were of HPLC grade purity.

Method of Preparation & Characterization:

polymeric nanoparticles of PLGA were
formulated by the using the technique oil-in-water emulsion/solvent evaporation
(Allam et al., 2000; Nair et al.,
2011). For the preparation of 5-fluorouracil
(5-FU) at a concentration of 1% (m/v), 10
mg of 5-fluorouracil was dissolved in
this solution of MEK (methyl ethyl ketone) 10 ml and myritol (250 mg) and mixed properly called solution-1. PLGA polymer
was dissolved in 25 ml of Dichloromethane and further added in solution-1, this
mixture was sonicated for 2 min to make a pre-emulsion. This mixer was added to a prepared
aqueous solution of PVC surfactant (100 mg in 50 ml) and sonicated for
10 min. This emulsion was concentrated for a volume of 10 ml using rotary
evaporator and found the concentration of 5-fluorouracil
1% (m/v) and called 5-fluorouracil
nanoparticles (5-FUNPs). This formulation was characterized on the basis of
particles size, zeta potential, TEM analysis & In-vitro drug release system
(Virk et al., 2017).


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